FP01-2. SQUAMOUS METAPLASIA AND BCL-6 IN ADENOIDS OF CHILDREN WITH OTITIS MEDIA WITH EFFUSION

Seung Geun Yeo, Seok Min Hong, Jae Yong Byun, Sung Wan Kim, Joong Saeng Cho, Chang Il Cha (Kyung Hee University, Korea)

Background: Deterioration in the mucosal barrier of adenoids makes the adenoids vulnerable to micro-organic infections, resulting otitis media with effusion. In this study, we evaluated the mucosal barrier, squamous changes of ciliated epithelium, IgA secretion and BCL-6 expression in adenoids. Materials and methods: Eighteen children with otitis media with effusion (OME group) and twenty children without any history of OME (Control group) were enrolled in this study. Hematoxylin and eosin stain of adenoid for squamous metaplasia, immunohistochemical study of adenoid for IgA and BCL-6, cytokeratin stain for evaluation of mucosal barrier and lateral view X-ray for adenoid size were performed. Mann-Whitney test was used in the analysis and data showing p value of less than 0.05 were considered significant. Results: The number of ciliated cells was decreased and squamous metaplasia was increased in the OME group (p<0.05). Deterioration of mucosal barrier was more detected in the OME group than control group (p>0.05). IgA secretion in the interfollicular and germinal center was increased in the OME group (p>0.05). BCL-6 in the germinal center was decreased in the OME group (p<0.05). There are no difference in adenoid size between OME group and control group. Conclusions: These results suggest that increase of squamous metaplasia and decrease of BCL-6 expression in adenoid may be concerned with increasing susceptibility of OME. However, the etiologic factor of OME and its clinical course are diverse and complex, its pathophysiology should be understood in association with other risk factors rather than considering that only adenoid problem is associated with the development of otitis media.

* Contact person email: yeo2park@khmc.or.kr