THE STUDY OF IMMUNOLOGICAL RESPONSE IN BONE RESORPTION IN CHOLESTEATOMA
B. Wang, X. Wang; W. Tong, T. Wang, T. Qin, J. Li
Dept. of Otolaryngology, 2nd Clinical Medical College, China Medical University,
Shenyang, 110003 P.R. CHINA.
Background: Up to now, the problem of lmmunological response in bone absorption in cholesteatooa is a subject to debate, it is still unclear
Objective: To investigate why the keratinized squamous epithelium cholesteaoma may promota greater amounts of bone breakdown and the resorption of bone in chiesteatoma.
Subjects: 18 experimental and 40 human cholesteatomas were used in this study.
Materials and Methods: 18 specimens of Experimental cholesteatomas induced by ligating the external auditory canal in 9 BALB/c mice and in 9 rats (Lew) within 6-12 months and 40 samples of cholesteatomas attached to bone undergoing erosion were obtained from 40 patients at tympano-mastoid surgery. Each of these specimens were studied with a histological and immunohistochemical technique with T3.T4.T6.T8. ckl. Monoclonal antibody (Dako Company, USA and ABC staining method (ABC kit Vetor INC, USA, and observed by light microscopy and transmission electron microscopy (T.E.M.). The same specimens of this contiguous bone edges were removed, decalcified and sections were made for histological study.
Results: The subpopulations of T-positive cell in cholesteatoma matrixes were compared with the mucosa and granulation tissue of chronic otitis media without cholesteatoma from the human subjects.
The matrix of Cholesteatoma contex with eroded bone were compared to without contact in eroded bone.
A large amount of LCs were always found in cholesteatoma matrix near the bone cortex and this contiguous bony edge resulted in T6,T8 cells response , induced inflammation and activity to promote and develop greater amounts of bone resorption and bone destruction. A lot of osteoclasts were displayed in the bone border.
Demonstrattion: Due to in the retractive pocket the desquamations of squamous epithelium, debris collected, bacteria and inflammation were developed antigen, especially the activated keratinocytes and T- cell reaponse generate cytokines, for example,the interleukin-1(IL-1) was produced and which activated osteoclasts, The significant resorption and destruction of bone were caused by the activated osteoclasts.